Products
A10 BioChip
A highly perfusable platform for the formation of thick and dense tissues
Features
-
Perfusion covered volume: 10mm x 6mm x 3mm.
-
70 porous microchannels (120μm OD, 80μm ID, ~7μm pores).
-
Perfusion for nutrients & waste exchange through the channels.
-
Drug distribution in the depth of the tissue, through the channels.
-
Top open chamber for easy cell loading.
Benefits
-
Easy to visualize tissue.
-
In vivo-relevant tissue morphologie and functionalities.
-
Improved prediction of in vivo compound response.
-
Save time and costs for drug and cosmetic development.
Skin
Colorectal cancer
Cosmetic model
Disease model
Hoechst / F-Actin
Ascorbic acid treated dermal fibroblast procollagen I levels in chip
Procollagen I (% of day 1)
Procollagen I (% of day 1)
Drug
Introduction
Drug
Introduction
Time (day)
Sculptra® PLLA treated dermal fibroblast procollagen I levels in chip
Procollagen I (% of day 1)
Procollagen I (% of day 1)
Drug
Introduction
Drug
Introduction
Time (day)
Skin dermis layer cultured in A10 BioChip.
Confocal image of dermal fibroblasts cultured in collagen I with perfusion through microchannels (top panel).
Anti-aging drugs tested on the in vitro skin model to show an increase in procollagen I expression after drug introduction compared to control (bottom panel).
0.3 mm
d0
d11
HCT116 cells cultured in A10 BioChip.
Bright field images of the cell loaded Matrigel after seeding (top left panel - d0) and after 11 days of culture (d11).
Top view of the tissue formed in the A10 BioChip on d11 (middle image). Confocal view of the thick CRC tissue on d11 (bottom).
0.3 mm
Toxicity model
Kidney
RPTEC cells cultured in A10 BioChip.
Confocal images of RPTEC cells culture around the chip channel (left image - scale bar: 1mm). When perfused, cells are polarized with cilia (apical surface) facing the inside of the chamber and the basolateral surface facing the surface of the tube (right images - scale bar: 100μm). Cells responded differently to exposure to low and high concentration of CdCl2.
300 μm
Toxicity assay.
RPTEC cells were exposed upon 48 hours to CdCl2 at low and high doses.
Confocal images of RPTEC cells. Cells responded differently to exposure to low and high concentration of CdCl2.
A low amount of CdCl2 is tolerated by cells, but higher doses will cause cell death (top). IL-6 analysis showed increase of inflammation marker (IL-6) at low CdCl2 concentration and none at high concentration.
C1 BioChip
A beginner-friendly platform for large tissue formation
Features
-
Perfusion covered volume: 10mm x 6mm x 2mm.
-
2 layers of porous microchannels with ~7μm pores
-
2 available channels dimension options:
-
1) 12 total microchannels, 600μm OD, 500μm ID, 400 μm distancing
-
2) 16 total microchannels, 500μm OD, 400μm ID, 200 μm distancing
-
-
Distribution of nutrients and drugs & waste exchange through the entire tissue.
-
Top open chamber for easy cell loading.
Benefits
-
Beginner friendly.
-
Easy to visualize tissue.
-
Cell and organoid on-chip culture.
-
Improved prediction of in vivo compound response.
-
Save time and costs for drug and cosmetic development.
400 μm
Fluorescent beads perfused at 40 μL/min in C1 chip
Matrigel was introduced into the chamber of a C1 chip. After polymerization, fluorescent beads in PBS were perfused into the channel at 40 μL/min.
400 μm
Perfusion and diffusion of FITC perfused in C1 chip.
Matrigel was introduced into the chamber of a C1 chip. After polymerization, FITC was perfused into the channel at 10 μL/min.
Disease model
Colorectal cancer
_edited_edited.jpg)
HCT116 cells cultured in C1 BioChip.
Bottom view of the tissue formed at the end of the culture period (top).
Tissue growth and viability was monitored on chip with Alamar Blue (top right graph) and cell death was monitored on chip with using a LDH assay kit (bottom right).
800 μm
Cell Growth (Alamar Blue)
Cytotoxicity (LDH)
BIOCOMPATIBILITY
Our BIO (material of the BioChips) supports over 90% cell viability in the in vitro cell culture process
Based on ISO 10993-5: 2009 Biological evaluation of medical devices compliant tests
SMALL MOLECULE DRUG ABSORPTION
Our BIO material used for BioChip printing exhibits minimal absorption of small molecule compounds such as Oxilaplatin, Ascorbic Acid, Cisplatin, 5-FU, and Dexamethasone, as confirmed by the results of High Pressure Liquid Chromatography (HPLC) testing.
Concentration (%)
Free drug concentration after 24 hrs. incubation on BIO sheet. (The data represent the means ± standard deviation of three independent experiments and are normalized to the control drug sample without a BIO sheet. Solutions of various small molecule compounds were utilized to incubate BIO printed sheet samples for 24 hours, after which they were collected and sent for HPLC testing.)
BioChip Accessory Kit
Our kits provide essential components to facilitate BioChip for culture of living cells, spheroids, organoids, and tissues.
Every accessory kit comprises chip holders, high-resolution imaging 6-well plates, needles, tubing, syringes, and all other essential components required for the culture.
We also provide syringe pumps and peristaltic pumps to support the perfusion culture.
